Lambda Physik Announces New Peptide-Analysis Application for UV-Star Laser

SANTA CLARA, CA --  Lambda Physik has announced that the UV-Star^TM, a tunable, all-solid state laser, is now available to use for absorption and laser induced fluorescence (LIF) analysis of DNA and amino acid peptide components. The amino acids tyrosine, phenylalanine, and tryptophan contain aromatic chromophores that absorb strongly at wavelengths below 310 nm. Because of this, it has been difficult to differentiate among these three amino acids via LIF analysis using the 266-nm fourth-harmonic output of an Nd:YAG laser. The design of the UV-Star enables absorption and LIF studies to be made between 282 and 310nm. The company says that at these longer wavelengths, the absorption of radiation by tyrosine and phenylalanine declines sharply relative to that of tryptophan. This allows tryptophan to be quantified by observing its LIF.

According to the Lambda Physik, the UV-Star is tunable over the wavelength range of 282nm to 310nm. Most standard 266nm Nd:YAG lasers can pump the UV-Star; when pumped with a diode-pumped solid-state laser, such as Lambda Physik's StarLine^TM, with a high-quality TEM₀₀ beam, the output of UV-Star is a near-diffraction limited Gaussian beam, says the company.

PTB Home